Aberrant development of pancreatic beta cells derived from human iPSCs with FOXA2 deficiency

<strong>Project manager(s)</strong><strong>: </strong>Essam M. Abdelalim Induced pluripotent stem cells (iPSCs) were generated from a patient with a heterozygous deletion of the short arm of chromosome 20 at bands p11.22 to p11.21 (~969 kb deletion), which contains only one gene, <em>FOXA2 </em>(<em>FOXA2^+/-</em>iPSCs) as well as healthy controls (Ctr1 iPSCs and Ctr2 iPSCs). <em>FOXA2^+/-</em>iPSCs were differentiated into different stages of beta cell development to understand the role of FOXA2 during pancreatic beta cell development as described in the article entitled "<strong>Aberrant development of pancreatic beta cells derived from human iPSCs with <em>FOXA2</em> deficiency" by Elsayed et al</strong>. The dataset represents RNA-seq data generated from pancreatic progenitors (PP2) and endocrine progenitors (EPs) derived from Ctr1 iPSCs, Ctr2 iPSCs, and three clones of <em>FOXA2^+/-</em>iPSCs. The file name is: Sample name _overall sample number_read direction_001 where: - PP2-Ctr 1: pancreatic progenitors (PP2) derived from Ctr1 iPSCs (healthy control 1) - PP2-Ctr 2: pancreatic progenitors (PP2) derived from Ctr2 iPSCs (healthy control 2) - PP2-FOX1, PP2-FOX2, and PP2-FOX3: pancreatic progenitors (PP2) derived from three different clones of patient-derived <em>FOXA2^+/-</em>iPSCs. - EP-Ctr1: endocrine progenitors (Eps) derived from Ctr1 iPSCs (healthy control 1) - EP-Ctr2: endocrine progenitors (Eps) derived from Ctr2 iPSCs (healthy control 2) - EP-FOX R1, EP-FOX R2, and EP-FOX R3: endocrine progenitors (EPs) derived from three different clones of patient-derived <em>FOXA2^+/-</em>iPSCs. - The RNA-Seq data were generated from two Ctr-iPSC lines and three FOXA2^+/-iPSC lines. - Read direction: R1 (Forward), R2 (Reverse).