Role of CRY1 in DNA end resection

CRY1 is a circadian clock core component. Data in our laboratory has linked this factor with controlling the activity of the DNA double strand break repair protein CtIP, affecting the process of DNA end resection. By using ChIP-seq approaches in the DiVA cell system, we can show that the absence of CRY1 increases DNA end resection, measured as the accumulation of RPA at the sites of cleavage of the enzyme AsiSI. Furthermore, this is due to an increase in the accumulation of the resection factor CtIP. Overall design: Chromatin inmunoprecipitation (ChIP-seq) of RPA, CRY1, CRY2 and CtIP in AsiSI-ER U2OS cells