Summary of in vitro and in vivo development following SCNT with antibiotic selectable marker-free cells.

*Donor nuclei from antibiotic selectable marker-excised cells with single-copy integration of transgene into different locus, or from untransfected cells (control), were used for nuclear transfer experiments.†BFF2 was evaluated as a genomic safe harbor according to the criteria articulated in a recently published study by Papapetrou et al.‡Values within one column are not significantly different (P>0.05).§Day-7 blastocysts were nonsurgically transferred to synchronized recipient cows with two embryos per recipient.¶No. pregnancies: number of pregnant recipients on day 90 d after embryo transfer/number of recipient cows.||Percentage of transferred recipients.