Single-nuclei RNA-seq (snRNA-seq) of postnatal hypothalamus of CRH-IRESCre (CRH-Cre) mice using a retrograde Connect-seq approach

We generated a single nuclei RNA-seq (snRNA-seq) dataset derived from the postnatal hypothalamus of CRH-IRESCre (CRH-Cre) mice using a retrograde Connect-seq approach. A snRNA-seq dataset of 1,533 cells were generated from the postnatal hypothalamus and the bed nucleus of CRH-IRESCre (CRH-Cre) mice with ages ranging from 6 to 14 weeks. The Connect-seq method combines the use of conditional neurotropic viruses that cross synapses with transcriptome analysis of single upstream neurons infected with the virus in order to identify the molecular identities in postnatal hypothalamic brain regions. <<>>