SUSTAINABLE PRODUCTION OF CAMPTOTHECIN IN THE SUSPENSION CULTURE OF THE ENDOPHYTE FROM NOTHAPODYTES NIMMONIANA

Sustainable production of camptothecin in the suspension culture of the endophyte from Nothapodytes nimmoniana， is disclosed. A novel high yielding and sustainable， camptothecin producing endophytes (A. alstroemeriae(NCIM 1408) and A. burnsii(NCIM 1409))and process for producing camptothecin from the said endophytes from Nothapodytes nimmoniana. The endophyte A. burnsii(NCIM 1409) is a high yield and sustainable， camptothecin producing endophyte (fungal strain) forming while colony mycelium which turns black during sporulation and forms aerial hyphae. The strain was able to produce 150-200 ??g/g DW biomass or 1.5-3 mg/L of CPT titer. The highest yielding endophyte (A. alstroemeriae (NCIM 1408) demonstrates a high yield of camptothecin up to 300-400 ??g/g DW biomass.