Additional file 5 of MetaPro: a scalable and reproducible data processing and analysis pipeline for metatranscriptomic investigation of microbial communities

Additional file 5: Table S1. Summary of Sequence Read Processing for Three Metatranscriptomic Datasets (NOD Mouse gut; Kimchi and Human Oral Biofilm) Processed by HUMAnN3, HUMAnN2, MetaPro and SAMSA2. This table reports the processing results from the four pipelines on samples from three different datasets. HUMAnN2 and HUMAnN3’s preprocessing tool concatenates paired reads into 1 single file and treats them as 2 separate reads. The NOD mouse samples are paired-end data, while the kimchi and human oral biofilm represent single-end sequence datasets. Unlike MetaPro and SAMSA2, HUMAnN3 and HUMAnN2 do not report transcripts but instead group proteins identified in their pipelines into gene families that are reported in the final column.