Expression data of ex vivo and stimulated p14 TE (day 8) and p14 TM (day 46)

The spectrum of pathogen-specific CD8+T cell functionalities, including their chemokine production profiles, remains incompletely defined. Here, we have employed microarray analyses to determine the gene expression profiles of lymphocytic choriomeningits virus- (LCMV-) specific effector (TE) and memory (TM) CD8+T cells in the established p14 chimera model; analyses of p14 TE and TM expression profiles were conducted directly ex vivo and after 3h TCR engagement using aCD3 and aCD28 antibodies (note that the present ex vivo p14 TE and TM data have previously been uploaded in the context of a related study [GSE38462] and are included here due to analyses now conducted together with stimulated p14 TE and TM data). Congenic LCMV Armstrong-immune p14 chimeras were generated in a staggered fashion to allow for concurrent sample collection at the effector and early memory stage. In brief, splenic CD90.1+ effector p14 TE (8 days post infection/dpi) and memory p14 TM (46 dpi) were magenetically pre-enriched from individual p14 chimeras (n=3-4) using an anti-PE selection kit (EasySep, StemCell Technologies), and further purified by FACS-sorting of CD8+CD90.1+ cells (FACSAria, BDBiosciences) achieveing >99% purity; each sorted sample was then divided into two aliquots and processed either for direct extraction of total DNA-digested RNA (ex vivo), or 3h stimulation with aCD3/aCD28 prior to RNA extraction (stimulated).