Tumor-associated neutrophil 1 precursors impair homologous DNA repair and promote sensitivity to PARP-inhibition

This dataset contains scRNA-Seq (SMARTSeq-2 and BD Rhapsody) data associated to our manuscript entitled "Tumor-associated neutrophil 1 precursors impair homologous DNA repair and promote sensitivity to PARP-inhibition".Access to data is Restriced. Please contact us for data access request.Contents:SMART-Seq2 (in vivo): tumor-associated PreNeu vs. mature neutrophilsSingle cells were FACS-sorted directly from three pooled, fresh ER⁺, highly proliferative (Luminal B, Ki-67 > 20%) breast tumors into: · Cluster 3 (PreNeu): CD45⁺ CD11b⁻ CD33⁻ CD16⁻ CD66b⁺ CD71⁺ CD117⁺ CD45RA⁺ · Cluster 2 (mature tumor-infiltrating neutrophils): CD45⁺ CD11b⁺ CD15⁺ CD66b⁺ LOX-1⁺ Clusters were defined by scRNA-seq and marker expression, and directly compared (Cluster 3 vs. Cluster 2) to delineate precursor-specific transcriptional programs. SMART-Seq2 (in vitro): cord-blood–derived neutrophil populationsHuman cord-blood mononuclear cells from a donor pool were cultured 4–5 weeks in MCF-7–conditioned medium to generate myeloid and neutrophil populations. Cells were sorted into PreNeu-like (CD71⁺ CD117⁺ CD45RA⁺ CD66b⁺) and mature neutrophil (CD11b⁺ CD15⁺ CD66b⁺) fractions, then processed identically with SMART-Seq2 for direct comparison to their in vivo counterparts and validation of subset transcriptomes.For SMART-Seq2 experiments, single cells were captured into 96-well plates, libraries prepared with SMART-Seq2 (NEBNext Single Cell/Low Input RNA Kit; 17 cycles cDNA, 11 cycles enrichment), dual-indexed, and sequenced on an Illumina NextSeq 500 (75 bp), yielding > 500 K mapped reads per cell. BD Rhapsody: CD45⁺ tumor infiltration profilingFrom one Luminal B ER⁺ breast tumor, CD45⁺ cells were enriched via magnetic microbeads and captured on the BD Rhapsody HT single-cell platform. Whole-transcriptome libraries were generated with the BD Rhapsody WTA Kit (8 cycles), sequenced on an Illumina NextSeq 2000 (P2 flow cell, XLEAP chemistry; ~8 000 reads/cell), and processed through the Seven Bridges Rhapsody pipeline against GRCh38, with QC, UMI counting, and clustering performed in Seurat.