Western Blot Analysis of KDM5C loss in WT and KDM5C-KO mES cells.

Western Blot analysis was conducted on whole cell lysates from WT mES and KDM5C-KO mES cells with anti-KDM5c. Cells were lysed with 2X Laemmli Buffer and were loaded at various concentrations on 8% acrylamide gels and probed with anti-KDM5C antibody which has been described previously in
Iwase, S., Brookes, E., Agarwal, S., Badeaux, A.I., Ito, H., Vallianatos, C.N., Tomassy, G.S., Kasza, T., Lin, G., Thompson, A., et al. (2016). A Mouse Model of X-linked Intellectual Disability Associated with Impaired Removal of Histone Methylation. Cell Reports 14(5):1000-9 (PMID: 26804915)

'WB SMCX KO cells_PonceauS' :Ponsceau S staining of the membrane after transfer

'WB SMCX KO cells_RawData' : anti-KDM5c western blot. Left Panel: High Exposure, Right Panel: Low exposure.
Left panel is horizontal inversion of the right panel.

Lane Information of right Panel and Ponceau S staining
Lane 1: Protein Ladder
Lane 2: KDM5C-WT mES (20ul)
Lane 3: KDM5C-KO mES (20ul)
Lane 4: Protein Ladder
Lane 5: KDM5C-WT mES (10ul)
Lane 6: KDM5C-KO mES (10ul)
Lane 7: Protein Ladder
Lane 8: KDM5C-WT mES (5ul)
Lane 9: KDM5C-KO mES (5ul)