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RNA-sequencing of macrophages after stimulation of SLAMF7

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NIAID Data Ecosystem2026-03-13 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE185509
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This study evaluated transcriptomic changes in monocyte-derived macrophages from healthy donors after stimulation with recombinant SLAMF7 or anti-SLAMF7 antibody, compared to macrophages without additional stimulation. Monocyte-derived macrophages were pre-incubated with IFN-g (10 ng/ml) for 24 hours, then stimulated anti-SLAMF7 (10 ug/ml) or recombinant SLAMF7 (1 ug/ml) for an additional 4 hours. Lysates were harvested for RNA-sequencing using the Smart-seq2 protocol. Transcript were pseudoaligned and quantified using kallisto. >>>Submitter states: The raw sequencing files (fastq) will be deposited on dbgap, per instructions from NIH <<<<
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2022-02-17
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