Nucleosome profiles in strains with different inter-NDR distances at HML

Heterochromatin is a complex structure comprised of nucleosome arrays that are bound by silencing factors. This composition suggests that certain configurations of nucleosome arrays are permissible for heterochromatic silencing. We tested this idea in S. cerevisiae by systematically altering the distance between heterochromatic Nucleosome Depleted Regions (NDRs), which is predicted to affect local nucleosome positioning by limiting how nucleosomes can be packed between NDRs. Consistent with this prediction, some inter-NDR distances led to poorly positioned nucleosome arrays within heterochromatin. Furthermore, these conditions that caused poor nucleosome positioning also led to defects in both heterochromatin stability and the ability of cells to generate and inherit a silenced transcriptional state. These findings strongly suggest that nucleosome positioning contributes to formation and maintenance of functional heterochromatin. We report nucleosome positions by using native Mnase-seq. The results strongly suggest that inter-NDR (nucleosomal depleted region) distance affects nucleosome positioning at HML. Genome-wide analysis of nucleosome positions in strains of interest, with a particular focus on nucleosome positioning at HML.