scRNA-seq of EZH2-knockout Memory B cells

To understand the transcriptional circuits controled by the histone methyltransferase EZH2 in memory B cells (MBC) scRNA-seq was performed on influenza-specific MBC that formed at an early day 14 or late day 39 time point. EZH2-knockout MBC were compared to CRE control MBC isolated from the spleens and mediastinal lymph nodes of mice. scRNA-seq of 4 sample across two time points comparing control and EZH2-deficient memory B cells