Primers and conditions for PCR amplification of FBXO7 cDNA and genomic DNA.
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The PstI restriction site was created by PCR using a mismatch primer. For Y52C and M115I amplification, the underlines in the primer sequence and enzyme recognition site indicate the mismatch nucleotide and polymorphic site, respectively. For cDNA cloning, the underlines in the primer sequence indicate the introduced HindIII, AgeI and XhoI restriction sites.
创建时间：
2015-12-02
