RNA-Seq is used to facilitate the identification of the BCR-ABL1- independent mechanism of resitance to imatinib using a human chronic myeloid leukaemia cell line model

The goals of this study were to compare two human chronic myeloid leukaemia cell lines. One is sensitive to the standard treatment imatinib whereas the other is resistant to imatinib. RNA-Seq was used to identify differentially expressed genes between the two cell lines. 5282 differentially expressed genes were identified, and using gene set enrichment analysis the TNFa signalling pathway via NF-?B was identified. Overall design: Differentially expressed genes were identified between two human chronic myeloid lukeaemia cell lines. RNA-Seq was performed using the Illumina Hiseq4000 platform at a depth of ~65 million reads per sample. Each cell line was run in triplicate.