Rice gene expression profiling in wild type and transgenic rice plants by over-expressing a class I HD-ZIP gene by Agilent custom 8X60K Microarray analysis

We designed an array based on the release 7 of Michigan State University (MSU) rice genome annotation database (http://rice.plantbiology.msu.edu). The array was used for investigating the differentially expressed genes after over-expression of the rice gene with locus name LOC_Os02g43330. The expression analysis was carried out using 14-day old whole seedlings from both japonica Nipponbare wild type (WT) and its transgenic plants by over-expressing the rice gene with locus name LOC_Os02g43330. Two biological replicates were carried out for both WT and transgenic plants.