Diffusive spreading across dynamic mitochondrial network architectures

Networks of physical units can vary from a stationary set of spatially-embedded links to a collection of mobile agents that undergo transient social interactions. In living cells, mitochondria form architectures that span across these regimes, transitioning between fragmented, partly connected, and highly fused structures depending on cell type and state. Diffusive transport of biomolecular components through these networks limits the heterogeneity of the mitochondrial population. Here we address the connection between dynamic network architecture and the rate of diffusive mixing through simulations and analytic models that incorporate fusion, fission, and rearrangement. We find that the material delivered from a source to the rest of the network depends on the network dimensionality and a balance of competing timescales for encounter, fusion, and diffusive dispersion. We extract morphological and dynamic parameters for mitochondrial networks in three human cell lines, demonstrating tha..., , # Data from: Diffusive spreading across dynamic mitochondrial network architectures

Dataset DOI: [10.5061/dryad.cjsxksnkb](10.5061/dryad.cjsxksnkb)

## Description of the dataset

#### Dataset Overview

For a comprehensive overview and the methodology used to generate and process all the data in this repository, please see the corresponding preprint at [https://arxiv.org/abs/2506.05643](https://arxiv.org/abs/2506.05643).

## Notes for Files

### Microscopy and Image Acquisition

All images were acquired using a Zeiss LSM 980 with Airyscan 2 laser scanning confocal microscope, equipped with 405-, 488-, 561-, and 639-nm laser lines and Fast Airyscan detector array. Images were acquired using an inverted 63×/1.4 numerical aperture oil objective. All live imaging was done in a humidified chamber at 37°C and in the presence of 5% CO2. Airyscan processing was performed using Zeiss ZEN Blue software version 3.7 (Carl Zeiss).

### *Holt_et_al_live_imaging.zip*

The compressed master directory ...,