BMDMs were generated via differentiation of bone marrow cells in culture media containing m-CSF for 7 day. Upon differentiation, BMDMs were left untreated or stimulated with LPS, IFNa, PGE2, LPS+PGE2,
Sensitivity testing of each cPCR and qPCR marker across a dilution series of genomic DNA. The proportion of detections out of four replicates is noted for each marker at each dilution level. Estimated
Assay validation is an essential component of disease surveillance testing, but can be problematic in settings where access to positive control material is limited and a safety risk for handlers. Here
Forward and reverse raw reads for all 31 removed samples from our study. These samples were removed because they failed (i.e. low number of reads), did not meet our curation cut-off defined in the met