Anterior Heart Field (AHF)-conditional Mef2c Knockout

We have performed conditional inactivation of mef2c in the anterior heart field (AHF) of mice and observed a phenotypic spectrum of outflow tract anomalies in the conditional mutant hearts. In an effort to identify misregulated genes in the outflow tracts of the mutants, we have performed RNA-Seq on outflow tract samples dissected from E10.5 mutant and wild-type embryos. There are four wild-type samples and four mutant samples.我们在小鼠的前心场（anterior heart field, AHF）中实现了mef2c的条件性灭活，并在条件性突变心脏中观察到流出道畸形的表型谱。为了鉴定突变体流出道中的异常调控基因，我们对从胚胎发育第10.5天（E10.5）的突变型与野生型胚胎中解剖获取的流出道样本开展了RNA测序（RNA-Seq）。本研究共包含4份野生型样本与4份突变型样本。