Periodic oscillations in mitochondrial function under oxidative stress. SOD2 concentrations of 0.013 mM
收藏DataCite Commons2024-01-09 更新2024-07-27 收录
下载链接：
https://figshare.com/articles/dataset/Periodic_oscillations_in_mitochondrial_function_under_oxidative_stress_SOD2_concentrations_of_0_013_mM/7117808
下载链接
链接失效反馈官方服务：
资源简介：
These oscillatory mitochondrial time series under conditions of oxidative stress are analyzed in detail in Kembro et al. 2018. Mitochondrial chaotic dynamics: Redox-energetic behavior at the edge of stability. Sci. Rep. (in press)Mitochondrial model used was described in the project overview and in detail (Kembro et al. 2013. Biophys J 104(2):332-343; Kembro et al., 2014. Front Physiol 5:257).<b>Analytical methods. </b>Numerical integration of the ME-R model equations was performed with MatCont 2.4 in MATLAB 7.1, until steady-state solutions were obtained (i.e., when the magnitude of each time derivative was -10). Time series with duration of at least 6e<sup>6</sup> ms were constructed by numerical integration of model equations. To allow transient states to vanish, the system was computed during a time lapse of 2 e<sup>8</sup> ms. All studies were performed using the parameter setting optimized in our previous work (Kembro et al. 2013. Biophys J 104(2):332-343; Kembro et al., 2014. Front Physiol 5:257), with ADPm = 0.01mM, i.e. consistent with energized mitochondria under state 4 respiration.The two .txt file represents the time reference and the time series of variables output of the model, in order from left to right column: 1) Mitochondrial Ca+;2) Mitochondrial ADP; 3) Membrane potential; 4)Mitocondrial NADH; 5) Mitochondrial H+; 6) Mitochondrial Phosfate, Pi; 7) Isocitrate; 8) a-ketoglutarate; 9) Succinyl CoA; 10) Succinate; 11) Fumarate; 12) Malate; 13) Oxaloacetate; 14) NADPH; 15) Mitochondrial superoxide; 16) Extramitochondrial superoxide; 17) Mitochondrial hydrogen peroxide; 18) Extramitochondrial hydrogen peroxide; 19) Mitochondrial GSH; 20) Extramitochondrial GSH; 21)Mitochondrial GSSG; 22) Mitochondrial TrxSH2; 23) ExtramitochondrialTrxSH; 24)Mitochondrial PSSGm; 25) Extramitochondrial PSSG.Original code of the model has been included<br>The parameter settings were:Model-simulated time series were calculated with 0.013 mM of SOD2, Shunt=0.04, SOD1 9.7 10<sup>-5</sup> mM. External superoxide perturbation: amplitude=1 10<sup>-7</sup> mM, period =30 s.<br>
提供机构：
figshare
创建时间：
2018-09-21
