RNA-seq of primary human keratinocytes : Effects of IL-20 on gene expression

In the present study, RNA-seq were performed To explore biological significance of IL-20 in the neuro-cutaneous signaling network of chronic itch. mRNA profiles were generated by deep sequencing, in triplicate, using MGISEQ-2000. We found that human keratinocytes responded to IL-20, resulting in enrichment of genes promoting itch and inflammation. IL-20 activation promotes cutaneous inflammation and itch, both leading into an aggravation of AD. This finding might help us to better understand the immune-neuro-modulatory mechanism in itch circuits Overall design: primary human keratinocytes were stimulated with IL-20 (500 ng/ml) or vehicle control. gene expression of these cells were investigated utilizing MGISEQ-2000.