Neutrophil aging exacerbates high fat diet induced metabolic alterations

In this work, we aimed to characterize how a high-fat diet affects neutrophil plasticity and aging in the bone marrow, as well as their infiltration into inflamed metabolic tissues. For the metabolic responses, we performed shotgun proteomics as reported here in physiology (wild-type mice, WT) and in mice with constitutively aged neutrophils (MRP8 driven conditional deletion of CXCR4; herein CXCR4fl/flCre+) or with constitutively fresh neutrophils (MRP8 driven conditional deletion of CXCR2; CXCR2fl/flCre+) following feeding with a High Fat Diet (HFD) (45% Kcal from fat) which promote the development of obesity.