Jennifer Bestman, Holly Cline (2011) CIL:37073, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is IMAGE #4 (hour 6) of a developing neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.
The cell was imaged at a 2 hour intervals over 8 hours and shows the development and morphological plasticity of the dendritic arbor and axon over time.

The cell was transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

本数据集包含来自白化非洲爪蟾（Xenopus laevis）神经索中发育中的神经元的第4张图像（第6小时）。该细胞在8小时内以2小时为间隔进行成像，展示了树突树和轴突随时间推移的发展与形态可塑性。在图像获取前约24小时，通过体内电穿孔法将编码eGFP的质粒DNA转染至细胞中。实验中，完整的活体蝌蚪被麻醉，置于玻片下，并通过定制构建的双光子显微镜直接穿透其透明皮肤进行成像。