RNA levels in wild-type and HIF1a-mutant MCF7 cells in normoxia (21% O2), and in hypoxia (1% O2) for 3 h or 24 h

We report the generation of CRISPR/Cas9-engineered MCF7 human breast cancer cells that lack functional HIF1a. We show that the HIF1a-dependent transcriptional response to hypoxia is severely impaired in HIF1a-mutant MCF7 cells. Overall design: RNA profiles of 2 cell lines (wild-type and HIF1a-mutant MCF7 cells ) in 3 conditions (grown at 21% oxygen, at 1% oxygen for 3 hours and at 1% oxygen for 24 hours) in triplicates, using Illumina HiSeq 2500