mRNA profiling after TAC/Sham surgery in NRON KO/wt mice

The long-non coding RNA NRON is enriched in cardiomyocytes where it localizes in the nucleus. Unexpectedly, NRON-overexpression in a transverse aortic constriction-induced hypertrophy mouse model exacerbated hypertrophy, whereas cardiomyocyte-specific NRON-deletion attenuated cardiac hypertrophy, thereby suggesting a novel regulatory role of NRON in the heart. Overall design: To gain insight into molecular processes affected by NRON KO in cardiac hypertrophy, we performed RNA sequencing of heart samples from WT and NRON KO mice 6 weeks after Sham and TAC surgery. Global gene expression was compared between three groups: WT mice exposed to Sham surgery (Sham WT), WT mice exposed to TAC surgery (TAC WT) and NRON KO mice exposed to TAC surgery (TAC KO).