Mass-spectrometry of cerebrospinal fluid (liquor) obtained from patients with hypoglossal schwannoma

Cerebrospinal fluid (liquor) samples (N = 44) have been derived from patients with rare tumor hypoglossal schwannoma that comprises about 5% of all non-vestibular schwannomas. Applying high resolution tandem mass-spectrometry 559 proteins were identified with high confidence (at least 2 peptides per protein, FDR <1%) liquor samples. After tryptic digestion, liquor samples were spiked with 148 stable isotopically labelled peptide standards (SIS). Their natural counterparts were mapped onto 111 FDA-approved protein markers. The samples were subjected to alkaline fractionation followed by scheduled multiple reaction monitoring (MRM) of resulted five fractions. The MRM\SIS dataset provides unique quantitative information on proteomic composition of hypoglossal schwannoma liquor samples.