Analysis of Ronin knockout related gene expression changes in mouse embryonic stem cells.. Analysis of Ronin knockout related gene expression changes in mouse embryonic stem cells.

Ronin knockout is embryonic lethal and Ronin knockout ES cells are not viable. Here we used CreER2; Roninflox/flox mouse ES cells to induce Ronin knockout by tamoxfien treatment in comparison to control Roninflox/flox cells to study Ronin knockout related transcriptional changes. Overall design: Roninflox/flox (control) or CreERT2; Roninflox/flox mouse ES cells were plated at a density of 100,000 cell per 10 cm2 and cultured in standard mouse ES cell medium. Cells were treated with 0.25 µM tamoxifen for 1.5, 3 or 6 days and total RNA was isolated to study gene expression changes.