Linker Amplification: Combination of genome (Pseudoalteromonas phage H105/1) and metagenome (Biosphere2 Ocean) datasets. marine metagenome

This dataset represents both clonal phage genome and wild virus metagenome data used to validate Linker amplification for quantitative metagenomics of wild viruses and other ultra-low concentration DNA samples. The project was an assessment of a DNA amplification method intended for metagenomic samples prior to next-gen sequencing. We tested the method by sequencing a single genome isolate (Pseudoalteromonas H105/1) and a single wild virus community/metagenome sample (Biosphere2 ocean). For each, the isolate and the virus community, we tested several parameters by varying amplification cycle numbers and a PCR reconditioning step. Marine phage was isolated from North Sea seawater at 1m depth, off the coast of Helgoland, Germany (54.17 N, 7.88 E); host Pseudoalteromonas sp. H105; collected Sep-1990, kindly provided by A. Wichels (AWI Helgoland). For the isolate, there are 30 treatments of the same original phage isolate plus 1 unamplified (31 data sets total) For the virus community sample, there are 8 treatments of the same original water sample plus 2 unamplified (10 data sets total).