Ceramides drive colorectal cancer metastasis through a revival stemness program [BulkRNAseq_InVitro]

We demonstrate that tumor-intrinsic ceramide metabolism is crucial in initiating colorectal cancer (CRC) metastasis, a process further enhanced by dietary fat intake Overall design: In vitro AKP organoids were treated with palmitic acid or carmofur, a ceramidase inhibitor, to test the effect of fatty acid and ceramide levels on the transcriptional changes of AKP organoids