Dataset of Piglet Testes Multiple Reaction Monitoring Profiling Mass Spectrometry of Lipids and Related Methods

<p>Multiple reaction monitoring (MRM) profiling is an exploratory method for surveying lipids. It is based on the use of ion transitions calculated using expected parent and product ions for various classes of lipids. The collection of ion transitions or MRM scans used is derived from lipids listed at the Lipid Maps structure database. </p>

<p>In this publication we make an MRM profiling data set available. This dataset consists of 42 samples and blanks. The MRM profiling method usually is performed as a two-step approach (discovery and screening steps) using the same amount of testis sample per animal sampled.</p>

<p>In the discovery step or first step, a comprehensive screening is performed using representative samples. Representative samples or pooled samples are prepared using individual samples aliquots to obtain one sample per experimental group. In this study, we had a control and four experimental groups depending on the perinatal nutrition regimen of the piglets. Control piglets sampled at birth were labeled as "zero-hour (ZH) group". Piglets from the stay on sow (SOS) group received ad libitum colostrum. The other three experimental groups were bottle-fed with 20% of their birth weight of colostrum (COL20), bottle-fed 20% of their birth weight with formula (PF20), and 20% birth weight formula supplemented with whey (PFW20). </p>

<p>In the screening step, only the MRMs found in the five composite samples which presented ion intensity higher than 30% compared to the ion intensity of a blank sample were selected. These MRMs were split into three lists (M1, M2 and M3) to be used for interrogating individual samples. In this step, the organic solvent used to dilute the samples was spiked with a mixture of deuterated lipids (Avanti EquisSPLASH cat#330731) containing one of two isotopically labeled lipid per lipid class considering phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylglycerol (PG), phosphatidylinositol (PI), ceramide (Cer), cholesteryl ester (CE), triacylglycerol (TG), and diacylglycerol (DG). Acylcarnitines (AC) and free fatty acids (FFA) were also profiled but no internal standards were added. </p>

<p>Therefore, the structure of the dataset published comprised (i) the correspondence of file names and sample identities, (ii) the discovery dataset (5 pooled samples and one blank sample) and the methods used for it, and (iii) the screening dataset with 42 samples, 1 blank without internal standards (blank no IS), and three blanks containing internal standards. The blanks with internal standards were collected at the beginning (blank IS), at the middle (blank ISb) and at the end of the run (blank ISc) and the methods used for it. </p>

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