Genomic differences between EM93 and S288C on chromosome I.

Labeled DNA from eight segregants originating from two EM93 tetrads was hybridized to the GENECHIP Saccharomyces cerevisiae Tiling 1.0R Array. The resulting hybridization profiles allowed us to estimate locations of potential polymorphic regions, deletions, and introgressions present in EM93. (A) Graph shows the hybridization intensities for one tetrad for chromosome I. Polymorphisms between EM93 and S288C are apparent in the hybridization profiles. (B) Close-up of the Saccharomyces paradoxus introgression region on chromosome I. The graph shows the region between nucleotide 178,000 and 193,000 (see also Figure S2 and Table S2 LSP2). Graphs were created using a moving average of the hybridization intensities of the probes that have a unique hit in the S288C genome with a window size of 500 probes in A and 200 probes in B.