Identifying crossover locations in Arabidopsis thaliana wild type Col x CLC, msh2 Col x CLC and msh2 Col x Ler F2 populations using genotyping by sequencing.

Genomic DNA from 189 wild type Col x CLC, 191 msh2 Col x CLC or 187 msh2 Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Serra et al 2018 PNAS). Sequencing data was analysed to identify crossovers as previously reported, using the TIGER pipeline (Rowan et al., 2015 G3).