Comparison of the Apparent kcat and KD Values For PCNA of Pol δ Assembliesa.

aData from the assays of Pol δ complexes on poly(dA)/oligo(dT) template/primers shown in Figure 1C were fitted into the hyperbola binding equation, v = vbasal+{Vmax•[PCNA]/(Kd+[PCNA])} where v is the velocity for dNMP incorporation; vbasal is the dNMP incorporation without PCNA; Kd is the apparent dissociation constant of PCNA. The apparent kcat was calculated as Vmax/E. The relative specific activities (relative kcat) were determined by taking Pol δ4 as 100.