RNA-seq of B16F10 lung metastases derived from APOE2 and APOE3 mice

Genetic variation in the APOE gene modulates melanoma progression. To identify tumor cell-intrinsic mechanisms underlying differences in metastasis between the APOE2 and APOE3 genotypes, we performed tail vein injections of luciferase and GFP-expressing (triple reporter) B16F10-shApoe melanoma cells into APOE2 and APOE3 knock-in mice. We performed RNA-Seq on lung metastatic nodules derived from these mice. This analysis revealed protein synthesis as a process that is enhanced by APOE2. Overall design: RNA-seq conducted on purified lung metastatic nodules derived from tail vein injections of B16F10-TR-shApoe cells into APOE2 mice (n=8) and APOE3 mice (n=5).