LPS-induced genome-wide RELA binding sites and H3K27ac changes in phraryngeal epithelial cells

Gram negative endotoxin Lypopolysaccharide (LPS) leads to a strong innate immune response through TLR4 signalling. This latter pathway activates cannonical NF-kB pathway, including its member RELA. Here, we want to investigate the RELA response in terms of DNA binding as well as epigenetic changes induced by LPS recognition. Overall design: Treatment of FaDu cells with LPS and examination of RELA genome-wide binding sites as well as H3K27ac mark by ChIP-seq before and after stimulation. One biological replicate was analyzed for each condition.