Whole genome expression microarray of cancer stem cells isolated from HT29 and HCT116 Human colorectal cancer cell lines. Whole genome expression microarray of cancer stem cells isolated from HT29 and HCT116 Human colorectal cancer cell lines

In the present study we have isolated and characterized cancer stem cells and non-cancer stem cells (bulk tumor cells) from high grade human colorectal cancer cell line HCT116 and low grade human colorectal cancer cell line HT29. For this study, cancer stem cells and non-cancer stem cells (bulk tumor cells) were isolated from HCT116 and HT29 human colorectal cancer cell line. For isolating cancer stem cells by FACS, CD44 and CD166 tagged with V450 and PE respectively were used. CD44+CD166+ was the cancer stem cell population and CD44-CD166- was designated as the non-cancer stem cell (bulk tumor cells) population for this study. RNA was isolated from the isolated cell populations and microarray was done to study the whole genome transcriptomic changes. Overall design: 4 x 44k Whole genome expression arrays of FACS sorted HT29 cancer stem cells (n=2) and HT29 non-cancer stem cells (n=2) using total RNA obtained from FACS sorted cells. 4 x 44k Whole genome expression arrays of FACS sorted HCT116 cancer stem cells (n=2) and HCT116 non-cancer stem cells (n=2) using total RNA obtained from FACS sorted cells.