Raw data files for McArthur et al., "Antigen mobility regulates the dynamics and precision of antigen capture in the B cell immune synapse"

Supporting raw data files for McArthur et al "Antigen mobility regulates the dynamics and precision of antigen capture in the B cell immune synapse". The study investigated how antigen mobility affects B cells' ability to capture antigens from presenting membranes, using planar lipid bilayers as membrane substrates. Bilayer fluidity and antigen mobility were characterized using fluorescence recovery after photobleaching and single particle tracking (Fig. 1, S2). The research examined how antigen mobility impacts B cell spreading and phospho-signalling (Fig. 2), calcium flux (Fig. 3), NF-κB and NFAT transcription factor activation (Fig. 4), antigen extraction quantity (Fig. 5), spatiotemporal kinetics of extraction (Fig. 6), trafficking to MHCII+ compartments (Fig. 7), and B cells' ability to discriminate between low/high-affinity antigens on bilayers with varying mobility (Fig. 8). The data collectively demonstrate that reduced antigen mobility enhances both the speed and selectivity of antigen extraction in the B cell immune synapse.