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MRSA ST398
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2013-11-04
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Approbation of the optimized MLSA. Approbation of the optimized MLSA method of pathogenic Borrelia burgdorferi sensu lato
The possibility of optimizing MLSA by using linked nucleotide sequences of two gene loci, recA and ospA, was previously shown on isolates of established species identity. However, this technique has n
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Oligonucleotides used for amplification and labeling of signature sequences and as fixed probes.
aExcess primer = X, Limiting primer = L. bF. tularensis subspecies tularensis yields amplicon of 307 bp, subspecies novicida and mediasiatica of 451 bp.
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Early and late strain type population isolates, year of isolation, and results of PCR and Sanger sequencing of target genes.
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