An alkaline-resistant beta-glucosidase produced by a silkworm gut symbiont, Sphingomonas paucimobilis ZJ1, contributes to host flavonoid detoxification and promotes host survival

Saponins from mulberry are degraded by bacteria gut symbiont of silkworm. Objective is to identify the saponins and degradation product, as well as compare sample QS (standard saponins extract in minimal medium + methanol) vs QP (degradation extracts bacteria, minimal medium + methanol). The analytical standard Kaempferol 3-O-β -rutinoside (K3GR, CAS 17650-84-9, Sigma-Aldrich, Germany) was purchased and all solvents were of UHPLC-grade quality. the folder contains the UHPLC-HRMS and MS/MS profiles of the standard K3GR and extracts QS/QP. The file Saponins_materials_methods_results.docx contains the parameters of the samples analysis and summary of the results.

桑树中的皂苷被家蚕肠道共生细菌降解。本研究的目的是识别皂苷及其降解产物，并比较样品QS（最小培养基中标准皂苷提取物+甲醇）与QP（降解提取物细菌，最小培养基+甲醇）的质量分数。分析标准为金丝桃苷3-O-β-芸香糖苷（K3GR，CAS 17650-84-9，Sigma-Aldrich，德国）已购买，所有溶剂均为超高效液相色谱级。该文件夹包含标准K3GR和提取物QS/QP的UHPLC-HRMS和MS/MS谱图。文件Saponins_materials_methods_results.docx包含了样品分析参数和结果摘要。