Transcriptomic assessments (RNA sequencing) in ovaries following treatments of gravid female zebrafish to an antibiotic mixture

As the use of antimicrobials during pregnancy and the perinatal period may lead to adverse outcomes such as miscarriages and newborn disease, the purpose of present study was to evaluate the influence of antibiotic exposure in offspring following treatments of gravid female zebrafish to an antibiotic mixture. For the F0 generation, adult zebrafish (AB strain) at sexual maturity (150 days post fertilization (dpf)) were selected for experimentation (50% male and 50% pregnant female). Given a significant change occurred in eggs production and F1 survival at birth in present study, combined with antibiotics bioaccumulated in ovary, we suspect that antibiotic exposure might impact the ovary of F0 generation zebrafish, and thus affect egg production and survival. Therefore, ovaries of the F0 generation were evaluated for transcriptional effects in different antibiotics mixture exposure (0, 1 and 100 ug/L) using a HTS approach coupled with advanced bioinformatic tools.