Methods S1 - Reversal of Apixaban Induced Alterations in Hemostasis by Different Coagulation Factor Concentrates: Significance of Studies In Vitro with Circulating Human Blood

Perfusion technique. Studies in perfusion devices have been useful to improve basic and clinical knowledge on the effectiveness of hemostatic strategies in transfusion medicine, including effects of platelet concentrates and rFVIIa. Aliquots of blood are perfused through annular chambers exposing damaged vascular segments, as thrombogenic substrata, at a fixed shear-rate. This supplemental file provides visual information on this methodology, from the extraction of the rabbit aorta to be used as thrombogenic substrata, to the final morphometric analysis on cross-sections of the perfused vessels. (EXE)