RNA-seq from myogenic satellite cells from a translational porcine model for Duchenne muscular dystrophy, heterozygous female carriers, and their healthy wild-type counterpart

Satellite cells (SCs), the stem cell population of skeletal muscle, are crucial for growth and regeneration, and their dysfunction is increasingly recognized as a contributing factor to Duchenne muscular dystrophy (DMD). DMD is a severe, X-linked disorder caused by DMD gene mutations, leading to loss of dystrophin expression in muscle tissue and a progressive muscle degeneration. Here, we provide RNA-seq data from wild-type (WT), dystrophin-deficient (DMD), and heterozygous (HET) pig SCs, representing a translational model for human DMD. Muscle biopsies were collected from fetal and 3-day-old animals, enzymatically digested, and subjected to magnetically activated cell sorting for SC purification. Purified cells were cultured under normal growth conditions in proliferation (PROL) and after induction to differentiate into multinucleated myotubes (DIFF), and total RNA was extracted for 3' mRNA-seq. Libraries were prepared using NexteraXT and sequenced on a NextSeq550Dx. After quality control and deduplication, reads were aligned to the Sus scrofa reference genome, and gene counts were generated with STAR. Parts of this data have been previously published in a larger study and are now made fully accessible as an independent dataset. By capturing stage- and genotype-specific transcriptional signatures of SCs, this dataset offers new insights into the molecular defects associated with dystrophin-deficiency and serves as a reference resource for future studies investigating DMD pathogenesis.