Transcriptomics analysis of Pichia pastoris strains engineered for enhanced branched-chain amino acids production

Transcriptomics analysis revealed that Ilv3 gene overexpression regulates the metabolic networks of branched-chain amino acid synthesis, the TCA cycle, methanol assimilation, and carbon-nitrogen co-utilization in Pichia pastoris. This study compared the global gene expression differences between the wild-type and engineered strains under methanol induction. Overall design: RNA-Seq analysis of P. pastoris control strain (HTX-33) and engineered strain (HTX33-ILV3) grown in minimal medium with 0.5% methanol. Three biological replicates per strain.