The ectomycorrhizal basidiomycete Hebeloma cylindrosporum undergoes early waves of transcriptional reprogramming prior to symbiotic structures differentiation

Illumina HiSeq2000 technology was used to generate mRNA profiles from Hebeloma cylindrosporum pre-infectious stages (2 and 4 days) compared to control mycelium. Pre-infectious and control mycelia were harvested after 2 and 4 days and used for RNA extraction. Reads of 150 bp were generated and aligned to Hebeloma cylindrosporum reference transcripts (http://genome.jgi-psf.org/Hebcy2) using CLC Genomics Workbench 8.5.1. Overall design: mRNA profiles from Hebeloma cylindrosporum pre-infectious and control mycelium were generated by Illumina HiSeq2000 sequencing (150bp). Three biological replicates were sequenced for Hebeloma cylindrosporum mycelium in contact for 2 and 4 days with Pinus pinaster seedlings as well as for control mycelium at the same time-points.