Homo sapiens Raw sequence reads. Homo sapiens

1) deep sequencing small RNAs in 48-paired colon rectal cancer (CRC) tissue samples using a bias reduction protocol we have developed previously, 2) identifying and evaluating the potential to use a panel of miRNAs to distinguish cancer tissues from adjacent normal tissues in CRC,3) characterizing the “true” signature miRNAs in CR or CRC tissues which lays the ground for manipulating their expression to study their biological or physiological roles in CR/CRC,4) , and evaluating the expression level of these signature miRNAs using miRNA q-PCR in CRC cell lines which are excellent tools for drugs screening/testing using tumor mouse model and correlating their dysregulation with mutants of several major transcription factors that involved in CRC.