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Ramazzottius claudii draft genome assembly

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Figshare2025-10-26 更新2026-04-08 收录
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https://figshare.com/articles/dataset/Ramazzottius_claudii_draft_genome_assembly/27061267/1
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Data associated with<b> Vecchi &amp; Stec.</b><b>Mitogenome of a new </b><b><i>Ramazzottius</i></b><b> species (Tardigrada: Eutardigrada: Ramazzottiidae) discovered in rock pools along with its Temperature and Desiccation-Related Proteins repertoire</b>.<br>Draft assembly of <i>Ramazzottius claudii </i>genome obtained of sequencing after WGA (REPLI-g Mini Kit Cat. No. 150023, Qiagen) and debranching with T7 Endonuclease I (Cat. No. M0302, New England Biolabs). Sequencing was performed on a Nanopore MinION with a R10 chemistry flowcell using fast basecalling. Assembly has been done with Flye v 2.9.3 and polished with medaka v 1.11.3 (-m r1041_e82_400bps_fast_g615).Library prep for Nanopore sequencing was done with Native Barcoding Kit 24 V14 (SQK-NBD114.24) using Native Barcode 01 NB01 (in the same flowcells samples with NB02 and NB03 were also run). NB sequences are reported below.<br>This draft genome should be used with caution as it may still contain contaminant sequences. The raw reads can be found in NCBI under BioProject PRJNA1082523, accession number SRR28173874.<br><br>NB01 F CACAAAGACACCGACAACTTTCTTNB01 R AAGAAAGTTGTCGGTGTCTTTGTGNB02 F ACAGACGACTACAAACGGAATCGANB02 R TCGATTCCGTTTGTAGTCGTCTGTNB03 F CCTGGTAACTGGGACACAAGACTCNB03 R GAGTCTTGTGTCCCAGTTACCAGG
提供机构:
Vecchi, Matteo
创建时间:
2024-10-31
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