Transcriptomes of human monocytes exposed to oxLDL ex vivo

Innate immune memory is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to microbial components such as Beta glucan or the BCG vaccine. Here we characterize the transcriptional events following oxidized low-density lipoprotein (oxLDL) duced trained innate immunity using RNA-seq data. Overall design: Primary human monocytes were exposed to oxLDL or media alone for 24 hours in culture. oxLDL was then removed and cells were allowed to differentiate to macrophages for an additional 5 days. RNA was collected at baseline, day 1, day 6 and upon 4 hour re-exposure to LPS at day 6. .