Hatching of Ae. aegypti eggs in brackish and fresh water.

Results are the mean numbers of eggs from three replicate experiments ± standard deviation that hatched into larvae at 48 h from 150 original eggs. Unpaired Student's t tests were performed to determine the significance of differences in the mean numbers of eggs hatching in brackish and fresh water for the corresponding original and reversal colonies (columns 1 vs 2 and 3 vs 4 respectively) in the three different experiments. The asterisks indicate that in every such comparison significantly more eggs hatched in fresh water compared to brackish water at pvs 3 and 2 vs 4 were not statistically significant with p>0.05. The original brackish and fresh water colonies were derived from Ae. aegypti collected in Kurunagar and Thirunelvely respectively in the Jaffna peninsula.Hatching of Ae. aegypti eggs in brackish and fresh water.