MANF deletion abrogates early larval Caenorhabditis elegans stress response to tunicamycin and Pseudomonas aeruginosa.

Microarray analysis was performed to evaluate the changes in gene expression that are associated with manf-1 deficiency with and without exposure to tunicamycin. RNA was isolated from four groups, (wildtype (N2) and manf-1 mutant (tm3603) L1 stage larve with and without 5 hours exposure to tunicmycin (5microgram/milliliter)) for transcriptomic profiles analysis本研究通过基因芯片分析（Microarray analysis），评估了伴随与不伴随衣霉素（tunicamycin）处理时，与manf-1基因缺失相关的基因表达变化。实验从四组L1期幼虫样本中分离RNA：分别为未处理的野生型（N2）、经5μg/ml浓度衣霉素处理5小时的野生型（N2）、未处理的manf-1突变体（tm3603），以及经5μg/ml浓度衣霉素处理5小时的manf-1突变体（tm3603），上述样本均用于转录组谱分析（transcriptomic profiles analysis）。