Transcription Factor Activity Regulating Macrophage Heterogeneity during Skin Wound Healing

We aimed to study chromatin accessibility for TF binding sites for inferring TF activity in monocytes and macrophages during skin wound healing. Monocytes and macrophages isolated from skin wounds on male C57BL6/J mice on day 3 and 6 post-injury, subjected to enzymatic digestion to obtain single cells and pooled from two mice per time point. Nuclei were processed using the 10x Chromium platform. Libraries sequenced on NovaSeq 6000 with paired-end reads. Fastq files were generated and demultiplexed into single cells using Cell Ranger software. Monocytes and macrophages isolated from skin wounds on male C57BL6/J mice on day 3 and 6 post-injury by magnetic sorting and nuclei was isolated and processed for scATACseq according to manufacturer’s instructions.