Efect of Arpp21 deficiency on TCR repertoire diversity. Efect of Arpp21 deficiency on TCR repertoire diversity

To assess the impact of Arpp21 deficiency on the TCR repertoire, we generated Arpp21-deficient mice from which we isolated CD4 T cells and subjected them to NGS sequencing. Overall design: From the spleens of age-matched mice, 7 of which were WT and 7 of which were Arpp21 KO, 6.8 million CD4 T cells were purified by FACS. Technical details: - Library preparation kit: Takara SMARTer Mouse TCR alpha-beta Profiling Kit - Sample QC method: Qubit and Tapestation - Library QC method: Qubit and Tapestation - Instrument: Illumina MiSeq - Read length: PE300